Subclinical infection is common in LCL and other forms of leishmaniasis, and can be detected by a lack of lesions or scars suggestive of healed CL, and lack of a history of chronic ulcer but a positive delayed type hypersensitivity (DTH) skin response to leishmanial antigen, or production of IFNγ by blood cells stimulated with Leishmania antigen, indicative of a functional cellular immune response [68,69,70]. Here, IFNG is linked to infection.