Immunohistochemical analysis revealed that the expression of mesothelin, an invading CRC cell marker,22 and snail2, an epithelial-mesenchymal transition marker,23, 24 was induced at the submucosal invasive area of ApcΔ716Trp53+/R270H adenocarcinomas, but it was not detected in the invasive area of ApcΔ716Trp53+/flox tumors (Supplementary Figure 1a). The gene discussed is MSLN; the disease is colorectal carcinoma.