It has been shown in 3D cultures that the activation of Wnt/β-catenin signaling together with the epidermal growth factor pathway can induce the tube-like structures of intestinal epithelial cells.34 Moreover, such a mechanism may increase the invasive growth of tumor glands into stromal tissues.35 We herein observed that p53R270H induced marked morphological changes of tumor organoids to complex glandular structures that correlated with increased invasiveness. This evidence concerns the gene EGF and neoplasm.