In this study, we analyzed ApcΔ716Trp53R270H/R270H tumor organoids because immunocytochemical staining showed a predominant p53 localization in the nucleus, whereas the nuclear accumulation was limited and cytoplasmic stabilization of p53 was not detected in the ApcΔ716Trp53+/R270H organoids (Supplementary Figure 3a). This evidence concerns the gene TP53 and neoplasm.