CD9 and cyst: In T vs. C, while five virulence factors (SPATR, PP2C, VP1, ROP5, AMA1) were upregulated in tachyzoite vs. cyst, the number of downregulated virulence factors was 10 (RON5, ROP16, RON2, GRA4, MIC3, ROP2A, GRA1, GRA6, GRA7, MIC6) (Figure 6B).