To decipher the cognate antigen driving T-cell activation in MS lesions, we analyzed T-cell reactivity in short-term TCL generated from different compartments of the same patient using autologous or alternatively an allogeneic HLA-matched BLCL stably transduced with seven human cMSAg (i.e., CNTN2, KIR4.1, MAG, MBP1, MOG, NFASC and S100B) [42, 43]. Here, S100B is linked to myeloid sarcoma.