The chromatin immunoprecipitation (ChIP) assay was carried out by using anti-FOXO1 antibody, and the results showed that FOXO1 could directly bind to the p27 promoter, as shown in Figure 4G. Significantly, ATG7 inhibition of FOXO1 protein expression was also observed in xenograft tumor tissues obtained from nude mice injected with UMUC3(shATG7) cells (Figures 4H and 4I). Here, ATG7 is linked to neoplasm.