FOXO1 and breast cancer: To determine whether FOXO1 was the downstream mediator responsible for ATG7 promotion of human BC tumorigenic growth, shRNAs specifically targeting FOXO1 (shFOXO1#1 and shFOXO1#2) were stably transfected into UMUC3 cells, T24 cells, and UMUC3(shATG7#1) cells, as shown in Figures 4A–4C.