Their effect on anchorage-independent growth ability was evaluated, and the results showed that knockdown of p27 increased the anchorage-independent growth ability of UMUC3(shATG7#1) cells and T24(shATG7#1) cells compared with that observed in scramble nonsense transfectants (Figures 2G–2I), suggesting that p27 is an ATG7 downstream effector responsible for the promotion of human BC tumorigenic growth. This evidence concerns the gene ATG7 and breast cancer.