To test the role of RUFY3 cooperation with FOXK1 in the progression of CRC, we injected LoVo/EGFP-N1, LoVo/EGFP-RUFY3-scr shRNA and LoVo/EGFP-RUFY3-FOXK1 shRNA cells expressing green fluorescent protein (GFP) into nude mice to examine liver metastasis. Here, FOXK1 is linked to colorectal carcinoma.