Rat lines bred for high anxiety-related behaviour (HAB) showed an increase in c-Fos expression in several areas of the brain including hypothalamus, habenula, and a decrease in other areas such as in the prelimic, infralimbic, cingulate cortex, when compared to rat lines bred for low anxiety-related behaviour (LAB), suggesting that specific areas of the brain are reactive and associating with anxiety [19, 58, 59]. Here, FOS is linked to Anxiety.