According to a systematic review, the sensitivity, specificity, and LR+ of AFP-L3 in HCC smaller than 5 cm in diameter ranged from 0.22 to 0.33, 0.93 to 0.94, and 4.63 to 30.8, respectively, with cut-off value of 10% and from 0.21 to 0.49, 0.94 to 1.0, and 8.06 to 45.1, respectively, with cut-off value of 15% [323]. This evidence concerns the gene AFP and hepatocellular carcinoma.