In support of the idea that Giardia in the foci are encysting, we noted significantly higher expression of the master transcriptional regulator Myb2, all enzymes of the GalNAc biosynthetic pathway (Figure 4), two known cyst wall proteins (CWP2 and CWP3), 17 HCMPs, and two EGF family proteins (Chen et al., 1996; Figures 2, 3, and Supplemental Table 4) in the in vivo foci. Here, EGF is linked to cyst.