To determine whether the increase in FXYD5 in AEC induces the NF-κB-dependent secretion of pro-inflammatory mediators, we infected cells with Ad-FXYD5, and 40 h after infection, determined the expression of FXYD5 (Figure 2A), phosphorylation of IκBα (Figure 2B), cellular levels of CCL2 mRNA (Figure 2C), and CCL2 and IL-6 levels in the culture media (Figures 2D,E). This evidence concerns the gene NFKB1 and infection.