In addition, both 5Aza (Fig. 5a–c) treatment and DNMT1 and DNMT3A knockdown (Fig. 5d–f) increased TIMP2 mRNA and protein expression and reduced DNA methylation of the TIMP2 promoter, suggesting that TIMP2 expression is down-regulated by DNA methylation in ovarian cancer cells. This evidence concerns the gene DNMT1 and ovarian carcinoma.