To determine the effect of different RGs on RT-qPCR analysis, the relative mRNA expression pattern of Pgc1α/PGC1α was examined in 3T3-L1 cells, PGAT from dietary and genetic models of murine obesity, as well as human SAT (Fig. 4 and Fig. S6). The gene discussed is PPARGC1A; the disease is obesity due to melanocortin 4 receptor deficiency.