KEAP1 and lung cancer: As shown in Figure 7b, we performed immunoprecipitation in the H1299 cells transfected with the plasmid expressing Flag-Keap1 and found that ESI indeed promoted the interaction between Keap1 and p62, and attenuated the binding of Keap1 to Nrf2, indicating a positive feedback loop of Nrf2-p62-Keap1 driven by ESI in lung cancer cells.