In contrast, RAD001 alone or in combination with docetaxel has significantly decreased P70S6K phosphorylation, SK1 mRNA and activity in ER-negative MDA-MB-231 and BT-549 breast cancer cells (Figs 2, S1) and hormone refractory prostate cancer cells PC3 (Fig. 2). Here, SPHK1 is linked to breast carcinoma.