Similarly, there was no correlation between NOTCH1 and canonical WNT target genes AXIN2 and DKK1 or stem cell genes previously identified to drive TPC growth in human rhabdomyosarcoma, including NANOG, POU5F1,or SOX2. Together, these results show that SNAI1 is a bona fide downstream target of NOTCH1 in ERMS and suggest non-overlapping roles for the NOTCH1/SNAI1 axis in regulating stem cell programs that drive tumor growth. The gene discussed is DKK1; the disease is rhabdomyosarcoma.