We found that NOTCH1 transcript expression highly correlated with GLI3 and, to a lesser extent, with GLI1; however, NOTCH1 expression did not correlate with either PTCH1 or PTCH2, or with Hedgehog-associated embryonic stem cell gene NANOG, suggesting that Notch and Hedgehog programs may regulate distinct self-renewal programs in rhabdomyosarcoma (Figures S4D and S4E). This evidence concerns the gene PTCH2 and rhabdomyosarcoma.