We also assessed whether NOTCH1 signaling was correlated with Hedgehog and canonical WNT/b-catenin signaling pathways previously shown to be associated with stemness and differentiation in rhabdomyosarcoma (Chen et al., 2014; Satheesha et al., 2016; Zibat et al., 2010). This evidence concerns the gene NOTCH1 and rhabdomyosarcoma.