Treponemal tests are directed against T pallidum proteins with high specificity; these include fluorescent treponemal antibody-absorption (FTA-ABS), Treponema pallidum hemagglutination assay (TPHA), TPPA and enzyme immunoassay (EIA), chemiluminescence immunoassay (CMIA), syphilis IgM antibody test (TP-IgM), TP Western blot test (TP-WB). The gene discussed is CD40LG; the disease is syphilis.