Previous work using 3D collagen matrices has, for example, reported higher invasiveness of breast cancer cells on areas that were not reorganised.44 Additionally, higher migration and invasion of breast cancer cells was found when ECM acquired a specific alignment.45 Thus, we explored the reorganisation of adsorbed FN by human fibroblasts in order to gain further insight on the different migratory response to FN conformation. Here, FN1 is linked to breast carcinoma.