To clearly determine the RASgrf1 alterations that occur during epileptic seizures, we detected the expression and methylation of RASgrf1 at different time intervals (1-day acute period, 10-day latent period and 45-day chronic phase) after acute epileptic seizures using bisulfite sequencing PCR (BSP), RT-PCR and western blot assays. The gene discussed is RASGRF1; the disease is Seizure.