A genome-editing RNP complex was formulated by mixing the Cas9 protein with two synthetic RNA oligonucleotides, a CRISPR targeting RNA (crRNA) duplexed to a trans-activating crRNA (tracrRNA) as described in “Materials” and “Methods.” The formed Cas9/RNP complex and HDR template, used to convert HbS to HBB*, were introduced by electroporation into HSPCs isolated from the SCD patient blood. This evidence concerns the gene RNPC3 and Schnyder corneal dystrophy.