HF diet increased the frequency of a variety of senescent markers in hepatocytes, including TAF, karyomegaly, mRNA expression of p16 (measured by p16 and eGFP RNA-ISH) and senescence‐associated β‐galactosidase (SA-β-Gal) activity (Fig. 3f–k and Supplementary Fig. 3e,f). This evidence concerns the gene CDKN2A and hydrops fetalis.