Here, infecting wild-type (WT) and IL-33-deficient mice (IL-33 KO) with L2-MHV3, we showed that the alarmin IL-33 ameliorated the L2-MHV3-mediated liver injury through the regulation of IFNγ expression, survival effect on immune cells, and neutrophil infiltration in viral hepatitis. Here, IL33 is linked to viral hepatitis.