NOS3 and endothelial dysfunction: It has been reported that isolated EV from plasma exert various effects on endothelial and trophoblast cells according to the physiological/pathological state of the pregnant woman.37,38 A decrease in expression and activity of eNOS bound to circulating nonpregnant EV has also been associated with cardiovascular diseases.39 Kao et al40 showed a significant increase in superoxide levels and impaired endothelial dysfunction in rat uterine arteries incubated with human preeclampsia plasma.