In this study, we aimed to evaluate the inhibitory effect of Wogonin on the invasion of CD133+ Cal72 human osteosarcoma stem cell, a critical step in metastasis of the tumour exhibited by the downregulation of MMP-9, and to elucidate the effects of Wogonin on the sphere formation ability of CSC to design a novel, natural, and safer anti-mobility and anti-invasion agent. The gene discussed is PROM1; the disease is osteosarcoma.