Mice deficient in either Diacylglycerol O-Acyltransferase 1 (DGAT1) or Lecithin-Cholesterol Acyltransferase (LCAT) are resistant to diet induced obesity and insulin resistance [48, 49] and these genes were not essential in any of the tested cell lines which makes them good potential pharmacological targets even if they are both associated to diseases in human (Congenital diarrheal disorder [50] and fish-eye disease [51] respectively). The gene discussed is DGAT1; the disease is obesity due to melanocortin 4 receptor deficiency.