To mimic the primary tumour, where only a small percentage of cells may express EMT-TFs4, we co-cultured GFP+HMLER-Control (HMLER-Ctrl) cells with tRFP+HMLER-Snail1 or Twist1 cells in a 1:1 or 10:1 ratio and performed migration assays. This evidence concerns the gene TWIST1 and neoplasm.