One study showed that melanoma patient DC matured with CD40L and IFNγ were often deficient in IL12p70 production but that this could be counteracted by addition of the two TLR ligands R848 and poly I:C [28] and that vaccination of melanoma patients with such CD40L/IFNγ/R848/poly I:C-matured DC loaded with neoepitope and shared peptides resulted in in vivo expansion of specific T cells [48]. The gene discussed is CD40LG; the disease is melanoma.