To further investigate the role of TRPC5-induced autophagy in breast cancer cells during chemotherapy, we transfected MCF-7 and MDA-MB 231 cells with full-length human TRPC5 and found that overexpression of TRPC5 significantly increased LC3-II levels and LC3 puncta formation (Fig. 2D and E). This evidence concerns the gene TRPC5 and breast cancer.