To the best of our knowledge, this is the first report on the different DENV serotypes in asymptomatic blood donners in KSA and the results showed an overall 5.5% prevalence of dengue by PCR, similar to the results of anti-dengue IgM antibodies, and the most prevalent serotype was DENV-2 and the lowest detected was DENV-3 serotype. The gene discussed is CD40LG; the disease is dengue disease.