In the present research, we found that in the high-fat/normal groups, whether in the F0 or F1 generation, transcripts associated with GnRH and Hippo signalling pathway were upregulated, suggesting that the biological processes and regulatory mechanisms participating in the GnRH and Hippo signalling pathways may represent potential targets for the treatment of male infertility in obesity induced by a high-fat diet. Here, GNRH1 is linked to obesity due to melanocortin 4 receptor deficiency.