ITGAX and neoplasm: In order to evaluate the immune response and determine infiltration of immune cells, HE and IHC staining were performed: by means of HE staining, the amount of immune cell infiltration in the tumor and surrounding tissues in different groups was observed; by IHC staining with F4/80, MHCII and CD11c, macrophages and dendritic cells were detected for the immune cell infiltrates evaluation as reported elsewhere [30].