Two OPTN mutants and three ANG mutants were used: OPTN E50K, found in glaucoma patients (Rezaie et al. 2002); OPTN E478G (Maruyama et al. 2010); ANG K17I; ANG K40I; and ANG P112L, found in ALS patients. This evidence concerns the gene OPTN and amyotrophic lateral sclerosis.