HSPB1 and Charcot-Marie-Tooth disease: In order to elucidate the pathomechanism of CMT in a more disease relevant in vitro model than immortalised cells, we established a cellular model in which primary motor neurons expressed Hsp27 mutations using 3rd generation lentiviral constructs expressing WT Hsp27 and the Ser135Phe, Pro39Leu and Arg140Gly mutations.