Sequential sampling of PLE 1 copy number after phage infection showed that PLE replication is low 10 minutes post-infection, but increases substantially 15 and 20 minutes post-infection (Fig 5B), which may indicate a switch from ICP1 replication (which occurs unperturbed early in infection (Fig 3C)) to PLE replication in infected cells. Here, ATP8B1 is linked to infection.