We confirmed ARRDC4 expression in peripheral blood mononuclear cells (PBMCs) derived from 30 EV71-infected HFMD children and 30 healthy candidates, which were age (HFMD 38.23±2.880 months, healthy control 40.67±2.156 months, P=0.5015) and sex (HFMD 60.0% male, healthy control 56.7% male, P=0.7934) matching. This evidence concerns the gene ARRDC4 and hand, foot and mouth disease.