The AS of CALD1, COL6A3, FN1, MAST2, LGR5 and ITGB4 were previously validated in colon cancer using RT-PCR24, while CLSTN1, AUP1, CTNND1, CALD1 and COL6A3 were shown to exhibit tumour-specific splice variants in colon, bladder and prostate cancers8. This evidence concerns the gene ITGB4 and colonic neoplasm.