PRP4K and familial pancreatic carcinoma: Previously, Carrigan et al. analysed AS in PDAC cell lines and demonstrated that 28 of 92 (~30%) spliceosomal genes had significantly decreased expression in pancreatic cancer compared with normal pancreas11; in our study, expression profiles for 18 of these 28 genes were identified and only three of them (SART1, CIRBP and HTATSF1) were significantly downregulated in PDAC (adjusted p < 0.05), while nine (DNAJC8, DHX9, DHX8, SLU7, RSRC2, MBNL2, NCBP2, PRPF4B and MBNL3) were significantly upregulated (adjusted p < 0.05) (Supplementary Fig. S6).