In the current work, it was found that NBD C6-Cer was efficiently taken up, and served as an intracellular substrate along with endogenous ceramides for glycosylation by GCS in cancer cells (Figs 2a and 3a), in dispersed cells of tissues (Figs 4a and 5a), and even in all tissues of live mice simultaneously (Figs 6 and 7). This evidence concerns the gene UGCG and cancer.