IL1B and alkaline phosphatase measurement: In parallel with these results, mRNA levels of NRLP3, ASC, and IL-1β in the pancreatic tissues of caerulein-injected SIRT1−/− mice were not attenuated by β-Lap (Supplementary Fig. 3D), suggesting that SIRT1 is critically required for the protective effects of β-Lap against AP.