Consistent with its tissue specific patterns, western blotting data showed that BRSK2 was expressed in PANC-1 cells, but was not detected in other tumor cell lines including liver cancer cell lines SK-Hep-1, HepG2, Focus, HuH-7, and SMMC-7721, lung cancer line H1299, osteosarcoma line U2OS, prostate cancer cell line PC-3, and another PDAC cell line AsPC-1 (Figure 1B). This evidence concerns the gene BRSK2 and liver cancer.