Histological analysis revealed that primary tumour morphology was unaltered when Gal-8 was silenced compared with the control cells (Figure 5c), and the expression of Gal-8 in primary tumours was still efficiently down-regulated in shGal-8.1 or shGal-8.2 conditions compared to the shCtrl, as determined by immunohistochemistry after tumour resection (Figure 5d–5e). This evidence concerns the gene LGALS8 and neoplasm.