As observed in Figure 2D, Boyden chamber assays were conducted, demonstrating that overexpression of PRMT5 substantially increased the number of migrated cells, whereas shPRMT5 knockdown showed quite opposite effect, pointing out the pivotal role that PRMT5 plays in the migratory ability of both PDAC and CRC. This evidence concerns the gene PRMT5 and colorectal carcinoma.