Real-time PCR and western blotting analysis revealed that overexpression of miR-196b-5p decreased, while silencing miR-196b-5p increased the mRNA and protein expression levels of SOCS1 and SOCS3, other three members of SOCS families were not affected by miR-196b-5p overexpression or downexpression, indicating that SOCS1 and SOCS3 may be the targets of miR-196b-5p in CRC cells (Supplementary Figure 1B and 1C and Figure 2B). The gene discussed is CISH; the disease is colorectal carcinoma.