Finally, we confirmed that T3 alone, without the induction of hypothyroidism via a PTU/LID, reduced hepatic CGL and CBS mRNA (Figure 4E), correlating with protein levels (Figure 4F), hepatic H2S production capacity (Figure 4G) and characteristic changes in hepatic metabolites, including cystathionine and cysteine (Figure S4C). This evidence concerns the gene CBS and hypothyroidism.