To determine if Akt2-S474 phosphorylation has a role in plasma membrane expression of Glut1 in cancer cells, we transiently co-expressed HA-Glut1 and Akt2-W80A or Akt2-W80A-S474A in a genetically engineered mouse lung cancer cell line (KP1 cells) (Choi et al., 2015). Here, SLC2A1 is linked to lung cancer.