In another double-blind, clinical trial that was done by Akram Kooshki et al. about the effect of symbiotics on inflammatory markers on patients with T2DM, has expressed consumption of symbiotic once a day for 8 weeks may decreased serum hs-CRP, IL-6 and TNF-α concentration, which are risk factors for CVD diseases [31]. Here, TNF is linked to type 2 diabetes mellitus.