It was used to reverse promoter methylation and thereby verify the epigenetic inactivation of ZAR1. ZAR1 methylated cell lines HEK, NSCLC cell lines A549, A427, H1299, H358, H322 and SCLC cell line HTB171 exhibited promoter demethylation with 5 or 10 μM Aza treatment, but unmethylated HCC-15 (NSCLC) remained unaffected (Fig. 4a). The gene discussed is ZAR1; the disease is small cell lung carcinoma.