CD4 and Opportunistic infection: Likewise, Shelburne et al. reported that predictive factors for Mycobacterium tuberculosis, Mycobacterium avium complex, and Cryptococcus neoformans-associated IRIS were antiretroviral drug naivety, active or subclinical opportunistic infection with high antigen burden before cART initiation, CD4 count <50 cells/μl and a rapid initial decrease in HIV VL in response to cART (40).