Importantly, when using PBMC from patients with melanoma in allogeneic coculture assays, we could observe a similar tendency of the PD-1/TIM-3 and PD-1/BTLA combinations to enhance proliferation, with TIM-3 being particularly effective in both CD4 and CD8 T cells (Figure S3 in Supplementary Material). The gene discussed is CD8A; the disease is melanoma.