Besides, the mechanism of PKM2 that regulate the cell-cycle progression of brain tumor cells have been manifested the fact that PKM2 interact with the spindle checkpoint Bub3 and phosphorylate Bub3 at Y20729, whether it exist in GC cells is worthy of further investigation, and the phenomena and experience acquired from the study are of critical significance to explore the potential mechanism of GC cells in our future study. Here, BUB3 is linked to gastric cancer.