Similar results were observed in IHC assays, moreover, Ki-67 expression and microvessel density (MVD) were substantially decreased in the NCI-N87-shPKM2 and BGC-823-shPKM2 groups, as compared to those in the control groups (Fig. 5C), which were consistent with the role of PKM2 in pancreatic cancer impairing tumor growth and decreased blood vessel formation in vivo25. This evidence concerns the gene PKM and neoplasm.