Several tumour suppressor genes were up-regulated after G9A depletion: cadherin (CDH1) a cell-to-cell adhesion glycoprotein with dysfunction contributing to cancer progression by increasing proliferation, invasion, and metastasis; dual specificity phosphatases (DUSP1, DUSP3 and DUSP5), that dephosphorylate MAP kinase and are frequently repressed in various cancer cells; Ep-CAM which contributes to cell-to-cell adhesion in certain cell contexts; GADD34 (PPP1R15A), a growth arrest and DNA damage-induced protein; Sprouty4 (SPRY4), an inhibitor of the Ras/MAPK signaling cascade. Here, SPRY4 is linked to cancer.