Genetic knockout (KO) experiments targeting DNA methyltransferases in human colon cancer cells (HCT116) reduced both DNA methylation and H3K9 methylation at the p16 locus, resulting in re-activation of the constitutively silenced p16/INK4A TSG and a decreased culture growth cell rate [130]. Here, CDKN2A is linked to malignant colon neoplasm.