For purification, EGFR was isolated from the insect cells after a 48-hour infection with an MOI of 10 of EGFR baculovirus via FLAG-purification from total cell lysates, which resulted in a band whose migration on an SDS-PAGE gel was consistent with full-length EGFR as shown by immunoblotting and Coomassie (Fig 1D and 1E). The gene discussed is EGFR; the disease is infection.